As so often before Bolt made a bad start, but this time he couldn’t make up the distance.
21 year old Christian Coleman, who beat Bolt in the semis, looked set for victory, but it was former world and Athletic champion Gatlin who took home the gold.
Gatlin won in 9.92 seconds, with Coleman taking silver in 9.94 and Bolt third in 9.95.
Gatlin’s win sparked a chorus of boos from a crowd unhappy with his doping past.
Bolt was seeking his fourth gold in the event before retiring next week after the sprint relay.